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KMID : 0380020100250020193
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 2 p.193 ~ p.198
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Effects of Jasmonic Acid-Cellulase Combined Elicitors on the Paclitaxel Production in Suspension Cultures of Taxus wallichiana Zucc
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Nguyen Ngoc Hoi
Hoang Van Luong
Nguyen Van Long
Vu Binh Duong
Byun Sang-Yo
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Abstract
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Cell cultures of Taxus wallichiana Zucc. were made to enhance the production of anticancer agent paclitaxel. In suspension cultures, the maximum cell growth rate in exponential growth phase was 0.14 day-1 which was correlated to 4.96 days of cell doubling time. The production of paclitaxel was non-growth associated. The paclitaxel production was started after the exponential growth phase and increased to declined phase where the maximum concentration was observed. Various elicitors were tested to enhance the production of paclitaxel. The combination of two elicitors of jasmonic acid and cellulase increased the production of paclitaxel 1.8 and 3.1 times compared to paclitaxel production by individual elicitor respectively.
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KEYWORD
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Taxus wallichiana Zucc, paclitaxel, jasmonic acid, cellulase
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